0.05mol⼀L磷酸盐缓冲液(pH7.0)如何配制？

若pH偏高或偏低，可通过改变二者的比例来加以调整，室温保存即可。

pH 0.05mol/L NaH2PO4(ml) 0.05mol/L Na2HPO4(ml)

5.7 93.5 6.5

5.8 92.0 8.0

5.9 90.0 10.0

6.0 87.7 12.3

6.1 85.0 15.0

6.2 81.5 18.5

6.3 77.5 22.5

6.4 73.5 26.5

6.5 68.5 31.5

6.6 62.5 37.5

6.7 56.5 43.5

6.8 51.0 49.0

6.9 45.0 55.0

7.0 39.0 61.0

7.1 33.0 67.0

7.2 28.0 72.0

7.3 23.0 67.0

7.4 19.0 81.0

7.5 16.0 84.0

7.6 13.0 87.0

7.7 10.5 89.5

7.8 8.5 91.5

7.9 7.0 93.0

8.0 5.3 94.7

25℃下0.1mol/L磷酸钾缓冲液的配制

pH 0.05mol/L K2HPO4(ml) 0.05mol/L KH2PO4(ml)

5.8 8.5 91.5
6.0 13.2 86.8
6.2 19.2 80.8
6.4 27.8 72.2
6.6 38.1 61.9
6.8 49.7 50.3
7.0 61.5 38.5
7.2 71.7 28.3
7.4 80.2 19.8
7.6 86.6 13.4
7.8 90.8 9.2
8.0 94 6.0

若pH偏高或偏低，可通过改变二者的比例来加以调整，室温保存即可。
pH
0.05mol/L
NaH2PO4(ml)
0.05mol/L
Na2HPO4(ml)
5.7
93.5
6.5
5.8
92.0
8.0
5.9
90.0
10.0
6.0
87.7
12.3
6.1
85.0
15.0
6.2
81.5
18.5
6.3
77.5
22.5
6.4
73.5
26.5
6.5
68.5
31.5
6.6
62.5
37.5
6.7
56.5
43.5
6.8
51.0
49.0
6.9
45.0
55.0
7.0
39.0
61.0
7.1
33.0
67.0
7.2
28.0
72.0
7.3
23.0
67.0
7.4
19.0
81.0
7.5
16.0
84.0
7.6
13.0
87.0
7.7
10.5
89.5
7.8
8.5
91.5
7.9
7.0
93.0
8.0
5.3
94.7
25℃下0.1mol/L磷酸钾缓冲液的配制
pH
0.05mol/L
K2HPO4(ml)
0.05mol/L
KH2PO4(ml)
5.8
8.5
91.5
6.0
13.2
86.8
6.2
19.2
80.8
6.4
27.8
72.2
6.6
38.1
61.9
6.8
49.7
50.3
7.0
61.5
38.5
7.2
71.7
28.3
7.4
80.2
19.8
7.6
86.6
13.4
7.8
90.8
9.2
8.0
94
6.0

取磷酸二氢钾0.68g,加0.1mol/L氢氧化钠溶液15.2ml，用水稀释至100ml，即得。其浓度是0.05mol/L